====== Laboratory of Medicinal Chemistry ====== ===== Head of the Laboratory ===== \\ {{:structure:labs:sin.jpg?nolink& |}}\\ **Aleksandr N. Sinyakov**,\\ Ph.D., Fellow of the Russia State Fellowship for Outstanding Scientists (1994-2003).\\ Phone: +7(383)363-51-73 \\ [[sinyakov@niboch.nsc.ru|e-mail]] \\ \\ \\ ---- ===== Staff ===== ^ ^ Name ^ Degree ^ Tel. ^ E-mail ^ Researcher ID ^ | 1. |Sinyakov N. Aleksandr |Ph.D. | 363-51-73 | [[sinyakov@niboch.nsc.ru |e-mail]] |[[http://www.researcherid.com/rid/H-1129-2013|H-1129-2013]] | | 2. |Ryabinin A. Vladimir |Ph.D. | 363-51-73 | [[ryabinin@niboch.nsc.ru |e-mail]] | | | 3. |Kostina V. Elena |Ph.D. | 363-51-72 | [[kostinaelena@mail.ru |e-mail]] | | | 4. |Maksakova A. Galiya |Ph.D. | 363-51-72 | [[galija@niboch.nsc.ru |e-mail]] | | | 5. |Pichko P. Natalya |Ph.D. | 363-51-73 | [[natalia_pichko@mail.ru |e-mail]] | | | 6. |Pozdnyakov G. Sergey |Ph.D. | 363-51-63 | [[pozdn426@niboch.nsc.ru |e-mail]] |[[http://www.researcherid.com/rid/H-1133-2013|H-1133-2013]] | | 7. |Venyaminova N. Larisa | | 363-51-72 | | | | 8. |Zaicev E. Dmitryi | | 363-51-72 | [[dmitry_z@mail.ru |e-mail]] | | | 9. |Homyakova V. Galina | | 363-51-72 | [[galj@yandex.ru |e-mail]] | | | 10.|Chizik I. Evgeniya | | 363-51-72 | | | | 11.|Kulmuhamedova M. Elena | | 363-51-72 | | | ---- ===== Research focus ===== * Development of new medical diagnostics methods. * Development of synthesis methods, investigation of physical, chemical and biological properties of minorgroove binders. * Synthesis of oligodeoxynucleotides and their derivatives for biochemical and medical investigation. ===== Main scientific results ===== * Methods of liquid-phase and solid-phase synthesis of single-stranded and double-stranded DNA-binding polyamides are developed. Physical and chemical properties of DNA-duplexes stabilized by minorgroove binders are investigated. * Modern methods of medical real-time PCR diagnostics are developed on basis of obtained data. * DNA-microchip methodology of diagnostics of orthopoxviruses and herpesviruses is developed. * Methods of synthesis of intermediates for real time PCR tests and DNA-microchip tests are developed. ===== Main publications 2011-2014 ===== - Sinyakov A.N., Ryabinin V.A. Microarray Diagnostics for Orthopoxviruses, filoviruses, arenaviruses and Type A Influenza Virus. in: Microarrays: Principles, Applications and Technologies. Rogers J.V. (ed.) Nova Science Publishers, Inc. NY. 2014, 147-186. - Ryabinin V.A., Kostina E.V., Sinyakov A.N., Compact microchip for typing of influenza A virus. Russian Journal of Bioorganic Chemistry, 2013. 39(3), 378-380. - Nikolaenkova E.B., Os’kina N.A., Savel’ev V.A, Samsonov V.A., Tikhonov A.Y., Ryabinin V.A., Sinyakov A.N. Synthesis of 4-(4-methoxyphenyl)-2,6-dinitro-benzaldehyde. Russian Journal of Organic Chemistry, 2013. 49(7), 1103-1105. - Novopashina D.S., Sinyakov A.N., Ryabinin V.A, Perruo L., Giovannangeli K., Ven’yaminova A.G., Butorin A.S. Oligo (2'-O-methyl ribonucleotides) and their derivatives. IV. Conjugates of triplex-oligo (2'-O-methylribonucleotides) ligands and the minor groove intercalators: synthesis, properties and applications. Russian Journal of Bioorganic Chemistry. 2013. 39(2), 159-174. - Kostina E.V., Ryabinin V.A., Maksakova G.A., Sinyakov A.N. A Second Generation Universal Microarray for Subtyping Influenza A Virus. Russian Journal of Bioorganic Chemistry. 2012. 38 (6), 599–604. - Kostina E.V., Ryabinin V.A., Agafonov A.P., Ternovoi V.A., and Sinyakov A.N. Microarray for Diagnostics of Human Pathogenic Influenza A Virus Subtypes. Russian Journal of Bioorganic Chemistry. 2011. 37(5), 644–646. - Ryabinin V.A., Kostina E.V., and Sinyakov A.N. Oseltamivir Resistance Depends on the Amino Acid at the 273rd Position of Neuraminidase of Influenza A Virus (H1N1) Circulating in the Human Population. Russian Journal of Bioorganic Chemistry. 2011. 37(5), 640–643. - Ryabinin V.A., Kostina E.V., Maksakova G.A., Neverov A.A., Chumakov K.M., Sinyakov A.N. Universal oligonucleotide microarray for sub-typing of influenza a virus. PLoS One. 2011. 6(4), e17529. - Shelkovnikov V.V., Loskutov V.A., Vasil’ev E.V., Shekleina N.V., Ryabinin V.A., Sinyakov A.N. New acid photogenerators based on thioxanthen-9-one sulfonium derivatives for detritylation in the oligonucleotide synthesis. Russian Chemical Bulletin. 2011. 60(3), 561-569. ===== Patents ===== - Kostina E.V., Sinyakov A.N. Ryabinin V.A., Maksakova G.A. Method of a choice of DNA-probes for microarray’s diagnostics, the biochip and a method of typing influenza virus A hemagglutinin and neuraminidase genes. Patent RF N.2470076, 2012. ===== Current grants ===== **Research is supported by Program of the Presidium of RAS** * N.24 «Fundamental bases of nanostructure and nanomaterial technologies»; Interdisciplinary Integration Projects of the Presidium of SB RAS. ===== Scientific equipment: ===== * Automatic synthesizers of DNA; * DNA-amplifiers; * HPL Chromatographs; * spotters for production of DNA microchips; * scanners of microchips; * the equipment for fine chemical synthesis.