Laboratory of Medicinal Chemistry

Aleksandr N. Sinyakov,
Ph.D., Fellow of the Russia State Fellowship for Outstanding Scientists (1994-2003).
Phone: +7(383)363-51-73
Email

• Development of new medical diagnostics methods.
• Development of synthesis methods, investigation of physical, chemical and biological properties of minorgroove binders.
• Synthesis of oligodeoxynucleotides and their derivatives for biochemical and medical investigation.

• Methods of liquid-phase and solid-phase synthesis of single-stranded and double-stranded DNA-binding polyamides are developed. Physical and chemical properties of DNA-duplexes stabilized by minorgroove binders are investigated.
• Modern methods of medical real-time PCR diagnostics are developed on basis of obtained data.
• DNA-microchip methodology of diagnostics of orthopoxviruses and herpesviruses is developed.
• Methods of synthesis of intermediates for real time PCR tests and DNA-microchip tests are developed.

1. Sinyakov A.N., Ryabinin V.A. Microarray Diagnostics for Orthopoxviruses, filoviruses, arenaviruses and Type A Influenza Virus. in: Microarrays: Principles, Applications and Technologies. Rogers J.V. (ed.) Nova Science Publishers, Inc. NY. 2014, 147-186.
2. Ryabinin V.A., Kostina E.V., Sinyakov A.N., Compact microchip for typing of influenza A virus. Russian Journal of Bioorganic Chemistry, 2013. 39(3), 378-380.
3. Nikolaenkova E.B., Os’kina N.A., Savel’ev V.A, Samsonov V.A., Tikhonov A.Y., Ryabinin V.A., Sinyakov A.N. Synthesis of 4-(4-methoxyphenyl)-2,6-dinitro-benzaldehyde. Russian Journal of Organic Chemistry, 2013. 49(7), 1103-1105.
4. Novopashina D.S., Sinyakov A.N., Ryabinin V.A, Perruo L., Giovannangeli K., Ven’yaminova A.G., Butorin A.S. Oligo (2'-O-methyl ribonucleotides) and their derivatives. IV. Conjugates of triplex-oligo (2'-O-methylribonucleotides) ligands and the minor groove intercalators: synthesis, properties and applications. Russian Journal of Bioorganic Chemistry. 2013. 39(2), 159-174.
5. Kostina E.V., Ryabinin V.A., Maksakova G.A., Sinyakov A.N. A Second Generation Universal Microarray for Subtyping Influenza A Virus. Russian Journal of Bioorganic Chemistry. 2012. 38 (6), 599–604.
6. Kostina E.V., Ryabinin V.A., Agafonov A.P., Ternovoi V.A., and Sinyakov A.N. Microarray for Diagnostics of Human Pathogenic Influenza A Virus Subtypes. Russian Journal of Bioorganic Chemistry. 2011. 37(5), 644–646.
7. Ryabinin V.A., Kostina E.V., and Sinyakov A.N. Oseltamivir Resistance Depends on the Amino Acid at the 273rd Position of Neuraminidase of Influenza A Virus (H1N1) Circulating in the Human Population. Russian Journal of Bioorganic Chemistry. 2011. 37(5), 640–643.
8. Ryabinin V.A., Kostina E.V., Maksakova G.A., Neverov A.A., Chumakov K.M., Sinyakov A.N. Universal oligonucleotide microarray for sub-typing of influenza a virus. PLoS One. 2011. 6(4), e17529.
9. Shelkovnikov V.V., Loskutov V.A., Vasil’ev E.V., Shekleina N.V., Ryabinin V.A., Sinyakov A.N. New acid photogenerators based on thioxanthen-9-one sulfonium derivatives for detritylation in the oligonucleotide synthesis. Russian Chemical Bulletin. 2011. 60(3), 561-569.

1. Kostina E.V., Sinyakov A.N. Ryabinin V.A., Maksakova G.A. Method of a choice of DNA-probes for microarray’s diagnostics, the biochip and a method of typing influenza virus A hemagglutinin and neuraminidase genes. Patent RF N.2470076, 2012.

Research is supported by Program of the Presidium of RAS

• N.24 «Fundamental bases of nanostructure and nanomaterial technologies»; Interdisciplinary Integration Projects of the Presidium of SB RAS.

• Automatic synthesizers of DNA;
• DNA-amplifiers;
• HPL Chromatographs;
• spotters for production of DNA microchips;
• scanners of microchips;
• the equipment for fine chemical synthesis.