D-LOOP FRAGMENT VARIABILITY OF MITOСHONDRIAL DNA IN SIBERIAN WHITE CRANE [Институт химической биологии и фундаментальной медицины]
ИХБФМ СО РАН » SB RAS Genomics Core Facility » Projects » Population genetics » D-LOOP FRAGMENT VARIABILITY OF MITOСHONDRIAL DNA IN SIBERIAN WHITE CRANE
D-LOOP FRAGMENT VARIABILITY OF MITOСHONDRIAL DNA IN SIBERIAN WHITE CRANE

D-LOOP FRAGMENT VARIABILITY OF MITOСHONDRIAL DNA IN SIBERIAN WHITE CRANE




The habitat maps and migration of a small dying sterkh Western population. They inhabit area downstream river Ob, in the particular subareas. The more numerous eastern populatin inhabit Yakutia. Some ornithologists suppouse these populations to be subspecies or de novo emerging species because of their long geographical isolation: the birds of Western population (<20) winter in Iran, whereas ones of Eeastern population (> 2500) do in China, on the lake Poyang.

Aim of the work: The genotypical comparison of Western and Eastern populations to test beginning of speciation hypothesis.

Applied problem: The answer the question «Could it use the Eastern population birds to restore the Western population,and, if so, could genetic markers be used to select donors, with genotype are the closest to collective genepool of the Western population?»

DNA samples studied:
17 sterkhs of The Oksky State Biosphere Reserve, from of whom 14 birds belong to the Eastern population with the rest to the Western population.
6 sterkhs had been living for the 80s. Their DNA samples were granted from collection of RAS Institute of Gene Biology.
5 birds of Eastern population.
1bird of Western population.



The marker used is highly variable region of the mitochondrial genome. The benefits of the selected marker are high variability (i.e. high resolution) and no «mixing» in a number generation by homologous recombination of chromosomes.









Results are presented in the form of a haplovariant network. Evident that the genepools of the populations significantly overlap.


So, the results showed that hypothesis of genetic subdivision in the beginning of speciation is not confirmed.



554496

samples have been analyzed since 2005


© Copyright 2024. ICBFM SB RAS

Яндекс.Метрика