Core Facility of Mass Spectrometric Analysis

Alexander A. Chernonosov,
Ph.D.
Phone: +7(383)363-51-74
Email

• Identification of genetic disorders detected in the neonatal period.
• Pharmaco-kinetics of drugs in pre-clinical and clinical studies: development of mass spectrometric methods for drugs detection, determination of drugs concentrations in biological fluids, calculation of the pharmaco-kinetic parameters.

• Methods of mass spectrometry determining for warfarin, warfarin alcohol and hydroxy warfarins in plasma samples of patients taking warfarin were developed and preliminary pharmaco-kinetics studies of warfarin metabolism were carried.
• Pharmaco-kinetic studies of metabolism of warfarin complex with arabinogalactan were carried out.
• Mass spectrometry method of amino acids quantification in ready meals (soups) has been optimized.
• Mass spectrometry methods of determining of nifedipine, glimepiride, alpha lipoic acid and a new antiviral drug NIOC-14 have been developed.
• The influence of genotype Vkorc1 on the pharmacokinetics of warfarin metabolism in the West Siberian populations of Caucasian ethnicity was determined.

1. Hvostov М.V., Chernonosov A.A., Tolstikova T.G., Kasakin M.F., Fedorova O.S., Duschkin A.V. Effect of Complexation with Arabinogalactan on Pharmacokinetics of ‘‘Guest’’ Drugs in Rats: For Example, Warfarin. BioMed Research International. 2013. V. 2013, Article ID 156381.
2. Kasakin M.F., Chernonosov A.A. Optimization of the method of mass spectrometric analysis for the determination of warfarin concentration in rat plasma. NSU vestnik. Series: biology, clinical medicine, 2013. 4.44-49.
3. Alekseeva I.V., Timofeeva N.A., Chernonosov A.A., Ivanova S.A., Bochan N.A., Fedorova O.S. Application of two-dimensional electrophoresis for proteomic studies of blood serum of patients with mental disorders. NSU Vestnik. Series: biology, clinical medicine. 2013. 4, 56-60.
4. Khvostov M.V., Tolstikova T.G., Chernonosov A.A., Fedorova O.S., Lifshits G.I. Pharmacokinetic alterations of warfarin in its clathrate with arabinogalactan. Asian Journal of Pharmaceutical and Clinical Research. 2012. 5(4), 251-252.
5. Abramova T.V., Kassakin M.F., Tarasenko Yu.V., Lomzov A.A., Koval V.V., Pyshnyi D.V., and Silnikov V.N. Synthesis and Properties of Nucleic Acid Methylene Carboxamide Mimics Derived from Morpholine Nucleosides. Russian Journal of Bioorganic Chemistry. 2012. 38(4), 400–411.
6. Chernonosov A.A. Eloquent metabolites. Science First Hand. 2012. Special Issue, 103-106.
7. Tkacheva N.A., Chernonosov A.A., Kokh N.V., Voronina E.N., Filipenko M.L., Lifshits G.I., Fedorova O.S. Study of the influence of genotype Vkorc1 C1173T and CYP2C9*2,*3 on the pharmacokinetic parameters of the warfarin metabolism. NSU Vestnik. Series: Biology, Clinical Medicine. 2011. 9(4), 155-160

• Chernonosov A.A., Koval V.V., Fedorova O.S. A method for determining the concentration of warfarin alcohol in plasma. Patent RF N.2486521, 2013.

Programs of the Presidium of RAS

• N.24 «Fundamental bases of nanostructure and nanomaterial technologies» (2013-2014)
  N.63 “Carbohydrate plant metabolites as a platform for the creation of nanostructured drugs”

• Mass spectrometers MALDI-TOF Autoflex Speed (Bruker Daltonics, Germany) and ESI
• LC / MS / MSD XCT (Agilent Technologies, USA); device for capillary electrophoresis (Agilent Technologies, USA);
• chromatographic systems (Series 1200) for the separation and analysis of peptides and for preparative separation of protein molecules (Agilent Technologies, USA).