Group of Microscopy

Elena I. Ryabchikova,
prof., D.Biol.Sci.
Phone: +7(383)363-51-63
Email

• Examination of the mechanisms of metal nanoparticles interaction with cells of pro- and eukaryotic organisms in vitro and in vivo.
• Studies of morphology and replication of viruses, bacteria and other microorganisms.
• Electron microscopic studies of structures in tears in patients with ophthalmic diseases.
• Examination of reproduction and antitumor activity of oncolytic viruses in vitro and in vivo.
• Visualization of macromolecular constructions and their interaction with cells.

•  Mechanisms of oncolytic activity of vaccinia virus recombinant producing apoptin were understood using carcinoma A431 xenografts in nude mice. Cytoplasmic effect of apoptin, enhancing vaccinia virus oncolytic effect was found.
• The structures were visualized and examined in lachrimal fluid of patients suffering from glaucoma, diabetic retinopathy and macular dystrophy. Morphological features of vitreous body and internal limiting membrane were described in patients with macular dystrophy.
• Plasmonic effect of gold nanorods under laser irradiation was examined and its damaging effect of melanoma B16 in mice was shown.
• Cells of hepatoma A-1 and peritoneal macrophages possess identical mechanisms of internalization of gold nanoparticles in vitro and in vivo. The cells use different mechanisms of endocytosis, specific to the type of cells, for internalization of nanoparticles.
• The method of preparation of palladium nanoparticles, identical to released from car motors in atmosphere, for application in studies with cell cultures has been elaborated. The penetration of palladium nanoparticles through the plasma membrane in MDCK culture cells and their accumulation in the nucleus was found. The macrophages internalized palladium nanoparticles by phagocytosis and clathrin-mediated endocytosis, and the nanoparticles accumulated in lysosomes. Thus, palladium nanoparticles inhaled from atmosphere, could damage epthelium of airways and accumulate in alveolar macropahges.
• Subsequent stages of the damaging effect of artificial ribonucleases on vaccinia virus, influenza, and bee paralysis virus were visualized. Development of viral envelope alteration and damage of viral nucleoid was observed.

1. Apartsin E.K., Buyanova M.Yu., Novopashina D.S., Ryabchikova E.I., Filatov A.V., Zenkova M.A., Venyaminova A.G. Novel multifunctional hybrids of single-walled carbon nanotubes with nucleic acids: synthesis and interactions with living cells. ACS Appl. Mater. Interfaces. 2014. 6(3), 1454-1461.
2. Kuhn J.H., Bào Y., Bavari S., Becker S., Bradfute S., Brauburger K., Rodney Brister J., Bukreyev A.A., Caì Y., Chandran K., Davey R.A., Dolnik O., Dye J.M., Enterlein S., Gonzalez J.P., Formenty P., Freiberg A.N., Hensley L.E., Hoenen T., Honko A.N., Ignatyev GM, Jahrling PB, Johnson KM, Klenk HD, Kobinger G, Lackemeyer MG, Leroy E.M., Lever M.S., Mühlberger E., Netesov S.V., Olinger G.G., Palacios G., Patterson J.L., Paweska J.T., Pitt L., Radoshitzky S.R., Ryabchikova E.I., Saphire E.O., Shestopalov A.M., Smither S.J., Sullivan N.J., Swanepoel R., Takada A., Towner J.S., van der Groen G., Volchkov V.E., Volchkova V.A., Wahl-Jensen V., Warren T.K., Warfield K.L., Weidmann M., Nichol S.T. Virus nomenclature below the species level: a standardized nomenclature for filovirus strains and variants rescued from cDNA. Arch Virol. 2014. 159(5), 1229-1237.
3. Grigor’eva A., Eremina A., Druzinin E., Chernykh D., Varvarinskiy E., Ryabchikova E. Diagnostic potential of the electron microscopic analysis of human lacrimal fluid Ophtalmosurgery. 2013. 4, 104-107.
4. Bychkov A.L., Ryabchikova E.I., Lomovsky O.I. Preparation of silver nanoparticles stabilized with the products of mechanoenzymatic hydrolysis of yeast cell walls. J. Intern. Scientific Publications: Materials, Methods and Technologies. 2013. 7(2), 439-446.
5. Grigoryeva A.E., Saranina I.V., Tikunova N.V., Safonov A., Timoshenko N., Rebrov A., Ryabchikova E.I. Fine mechanisms of the interaction of silver nanoparticles with the cells of Salmonella typhimurium and Staphylococcus aureus. Biometals. 2013. 26(2), 479-488.
6. Kuhn J.H., Bao Y., Bavari S., Becker S., Bradfute S., Brister J.R., Bukreyev A.A., Caı` Yı´ngyu´n, Chandran K., Davey R.A., Dolnik O., Dye J.M., Enterlein S., Gonzalez J.-P., Formenty P., Freiberg A.N., Hensley L.E., Honko A.N., Ignatyev G.M., Jahrling P.B., Johnson K.M., Klenk H.-D., Kobinger G., Lackemeyer M.G., Leroy E.M., Lever M.S., Lofts L.L., Mu¨hlberger E., Netesov S.V., Olinger G.G., Palacios G., Patterson J.L., Paweska J.T., Pitt L., Radoshitzky Sh.R., Ryabchikova E.I., Saphire E.O., Shestopalov A.M., Smither S.J., Sullivan N.J., Swanepoel R., Takada A., Towner J.S., van der Groen G., Volchkov V.E., Wahl-Jensen V., Warren T.K., Warfield K.L., Weidmann M., Nichol S.T. Virus nomenclature below the species level: a standardized nomenclature for laboratory animal-adapted strains and variants of viruses assigned to the family Filoviridae. Arch. Virol. 2013. 158(6), 1425-1432.
7. Olejnik J., Alonso J., Schmidt K.M., Zhen Yan, Wei Wang, Marzi A., Ebihara H., Yang J., Patterson J.L., Ryabchikova E., Mühlberger E. Ebola virus does not block apoptotic signaling pathways. Journal of Virology. 2013. 87(10), 5384-5396.
8. Zonov E.V., Voronina E.I., Zenkova M.A., Ageeva T.A., Ryabchikova E.I. Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice. Exp. Oncol. 2013. 35 (1), 30-36.
9. Babkin I.V., Tyumentsev A.I., Tikunov A.Yu. Kurilshikov A.M., Ryabchikova E.I., Zhirakovskaya E.V., Netesov S.V., Tikunova N.V. Evolutionary time-scale of primate bocaviruses. Infection, Genetics and Evolution. 2013. 14, 265–274.
10. Bychkov A.L., Ryabchikova E.I., Korolev K.G., Lomovsky O.I. Ultrastructural changes of cell walls under intense mechanical treatment of selective plant raw material. Biomass and Bioenergy. 2012. 47, 260-267.
11. Fedorova A.A., Goncharova E.P., Ryabchikova E.I., Vlasov V.V., Zenkova M.A. Novel amphiphilic compounds effectively inactivate the vaccinia virus. FEBS Lett. 2012. 586(11), 1669-1673.
12. Ismagilov Z.R., Shikina N.V., Mazurkova N.A., Tsikoza L.T., Tuzikov F.V., Ushakov V.A., Ishchenko A.V., Rudina N.A, Korneev D.V., and Ryabchikova E.I.., Synthesis of Nanoscale and Study of the Effect of Their Crystal Structure on Single Cell Response. The Scientific World Journal, v. 2012, Article ID 498345, 14 pages, 2012.
13. Ismagilov Z.R. Shikina N.V., Bessudnova E.V., Korneev D.V., Ishchenko A.V., Chesalov Y.A., Vladimirova A.V., Ryabchikova E.I. The Effect of Chemical Treatment Conditions of Titanium Dioxide Sols on Their Dispersion and Cytotoxic Properties. Chem. Engineering Transact. 2012. 27, 241-246.
14. Olejnik J., Ryabchikova E., Corley R.B., Mühlberger E. Intracellular Events and Cell Fate in Filovirus Infection. Viruses. 2011. 3, 1501-1531.
15. Fedorova A.A., Azzami K., Ryabchikova E.I., Spitsyna Yu.E., Silnikov V.N., Ritter W.J. Gross H.J., Tautz J., Vlassov V.V., Beier H., Zenkova M.A. Inactivation of a non-enveloped RNA virus by artificial ribonucleases: Honey bees and Acute bee paralysis virus as a new experimental model for in vivo antiviral activity assessment. Antiviral Research. 2011. 91, 267–277.
16. Patutina O., Mironova N., Ryabchikova E., Popova N., Nikolin V., Kaledin V., Vlassov V., Zenkova M.Inhibition of metastasis development by daily administration of ultralow doses of RNase A and DNase I. Biochimie. 2011. 93, 689-696.

RFBR (Grants of the Russian Foundation for Basic Research)

• N.13-04-01076 «Mechanisms of selective reproduction of vaccinia virus in tumor cells» (2013-2015)

Interdisciplinary Integration Project of the Presidium of SB RAS

• N.57 “Nanobiosafety: effects of nanoparticles on different biological organization levels – from molecules to organism” (2012-2014)

Program of the Presidium of RAS №5 “Basic Science for Medicine” (2013-2015)

• N.35 “Search for diagnostic and prognostic markers of primary open-angle glaucoma in tears”

• Machine for histological processing “Tissue-Tek” (Sakura Finetek Inc., USA);
• microtome for preparation of paraffin sections RM 2255 (Leica, Germany);
• light microscope Leica DM 2500 supplied with digital camera Leica DFC420 C (Leica, Germany);
• vaccum evaporation device JEE-420 (Jeol, Japan);
• Ultramicrotome for preparation of semithin and ultrathin sections Leica EM UC7 (Leica, Germany);
• transmission electron microscope of high resolution JEM 1400 (Jeol, Japan), equipped with built-in digital camera and side-mounted digital camera Veleta (SIS, Germany).