Laboratory of Genetic Diagnostics

Subbotovskaya Anna Igorevna
Ph.D.
Phone: (383) 330-76-66
Email

• Study of genetic associations prognostic value (polymorphic variants of genes of hemostasis, fibrinolysis, endothelial dysfunction, folate cycle) in diagnosis of thromboses of various localization.
• Development of new methodological approaches for laboratory diagnosis of endothelial dysfunction based on genetic testing and research of endothelial proteins with an estimate of the functional state of the hemostatic system as one of the leading factors in the development of thromboses of various localizations.
• Investigation of role of genetic factors in formation of organism's response to application of indirect anticoagulants (Varfarin) in patients with venous and arterial thrombosis after cardiac surgery.
• Study of prevalence and species specificity of pathogens carried by ticks in Novosibirsk region, development of optimal diagnostics and prevention of diseases transmitted by Ixodes ticks.
• Study of changes of level of extracellular blood plasma DNA in patients with Ixodes tick borreliosis depending on nature of the disease and characteristics of formation of an immune response against a background of acute and chronic disease course.

• Polymorphic variants of genes of hemostasis, fibrinolysis, endothelial dysfunction, folate cycle as prognostic markers of thrombosis of different localizations among West Siberian residents are studied. Use of proposed modern diagnostic standards of tromboses treatment an significantly reduce the rate of recurrent stroke and pulmonary embolism. [Tsvetovskaya G.A. et al., International journal of applied and fundamental research, 2009. 2, 22].
• Diagnostic standards for identification of thrombophilia genetic risk factors for women of reproductive age in Western Siberia are developed. [Tsvetovskaya G.A. et al, Fundamental Research. 2010. 10, 72].
• Use of personalized selection of warfarin dose , an anticoagulant dosing regimen based on preliminary genetic testing, and maintaining required level of anticoagulation with laboratory monitoring of therapy (analysis of indicators of international normalized ratio - INR) reduce the induction period of the drug and its side effects.
• It is proved that endothelial dysfunction, which determines the inefficient fibrinolysis, in combination with thrombophilia risk genes determines the basis for realization of predisposition to the disease process. Despite differences in the mechanisms of arterial and venous thrombosis, a number of common polymorphic genes for these pathologies are revealed.
• The study of ticks infected with borrelia in Novosibirsk Sientific Center showed that B. garinii is most frequently detected in ticks. In recent years number of ticks infected with B.miyamotoi increased, and at the same time there are two types of Borrelia - B. miyamotoi and B.afzelii.
• The study of extracellular DNA concentration in plasma and in association with the blood cells surface in healthy individuals and patients with erythema and nonerythema form of Ixodes tick-borne borreliosis showed that there are the differences in concentration of extracellular blood DNA, which determines the form of disease and the nature of immune response.

1. Livanova N.N., Livanov S.G., Fomenko N.V., Rar V.A., Tkachev S.E., Borgoyakov V.Yu., Panov V.V. Modern state of the natural foci of human transmissible infections. Dynamics of ecosystems of Novosibirsk Akademgorodok. Ed. acad. Zhimulev I.F. SB RAS publishing house. Novosibirsk. 2013, 350-357.
2. Borgoyakov V.Yu., Tkachev S.E., Chikova E.D. Spread the infestation of ticks and tick-borne encephalitis virus and Borrelia in a wooded park of Akademgorodok. Dynamics of ecosystems of Novosibirsk Akademgorodok. Ed. acad. Zhimulev I.F. SB RAS publishing house. Novosibirsk. 2013, 358-359.
3. Tkachev S.E., Borgoyakov V.Yu., Panov V.V., Livanova N.N. Genetic diversity of tick encephalitis virus. Dynamics of ecosystems of Novosibirsk Akademgorodok. Ed. acad. Zhimulev E.F. SB RAS publishing house. Novosibirsk. 2013, 366-371.
4. Bondar A.A., Cortese R., Bryzgunova O.E., Rykova E.Yu., Zaripov M.M., Voitsitskii V.E., Yarmoschuk S.V., Chikova E.D., Permyakova V.I., Ponomareva A.A., Yurmazov Z.A., Usinin E.A., Slonimskaya E.M., Cherdintseva N.V., Petronis A., Vlassov V.V. Search for epigenetic markers of prostate cancer in plasma circulating DNA. Siberian oncological journal. 2013. Supplement 1, 17-18.
5. Moskalensky A.E., Yurkin M.A., Konokhova A.I., Strokotov D.I., Nekrasov V.M., Chernyshev A.V., Tsvetovskaya G.A., Chikova E.D., Maltsev V.P. Accurate measurement of volume and shape of resting and activated blood platelets from light scattering. Journal of biomedical optics. 2013. 18(1). p 17001.
6. Tsvetovskaya G.A, Chikova E.D., Koch N.V., Lifshits G.I., Morozov V.V., Novikova Ya.V. Modern methods of laboratory diagnostics in prevention of repeated thromboses. Fundamental Research. 2012, 10 (2), 353-355.
7. Semenov D.V., Baryakin D.N., Brenner E.V., Kurilschikov A.M., Vasiliev G.V., Bryzgalov L.A., Chikova E.D., Richter V.A. Unbiased approach to profile the variety of small non-coding RNA of human blood plasma with massively parallel sequencing technology. Expert Opin. Biol. Ther. 2012. 12(1), 43-51.
8. Livanova N.N., Borgoyakov V.Yu., Livanov S.G., Tikunova N.V., Fomenko N.V. Characteristic of natural foci of tick-borne borreliosis in Novosibirsk scientific center and Novosibirsk region (Russia). Siberian Journal of Medicine. 2012. 111(4), 20-23.
9. Shkoda O.S., Chikova E.D., Fomenko N.V., Vlassov V.V., Laktionov P.P., Rykova E.Yu. Interrelation of blood circulating DNA with features and character of the immune response at patients with tick-borne borreliosis at early stages. Siberian Journal of Medicine. 2012. 111(4), 39-41.
10. Chikova E.D., Tsvetovskaya G.A., Patrushev A.Yu., Morozov V.V., Shevela A.I. Hypocholesteremia - as criterion of the prediction in surgical practice. Fundamental Research. 2012. 8-1, 174-179.
11. Tkachev S.E., Borgoyakov V.Yu., Livanova N.N., Panov V.V. The occurrence of tick-borne encephalitis virus genotypes and subgenotypes on the territory of Novosibirsk scientific center. Siberian Journal of Medicine. 2012. 111(4), 41-44.
12. Borgoyakov V.Yu., Vaskova A.A., Chikova E.D., Fomenko N.V. Variation in infection of ixodes ticks by borreliosis and tick-borne encephalitis virus IN NSC SB RAS during 2009-2011. Siberian Journal of Medicine. 2012. 111(4), 52-54.
13. Fomenko N.V., Spherling M.M., Borgoyakov V.Yu., Epichina T.I., Romanova E.V. A comparative analysis for identifying DNA and antiborrelia antibodies. Siberian Journal of Medicine. 2012. 111(4), 61-64.
14. Konokhova A.I., Yurkin M.A., Moskalensky A.E., Chernyshev A.V., Tsvetovskaya G.A., Chikova E.D., Maltsev V.P. Light-scattering flow cytometry for identification and characterization of blood microparticles. J. Biomed. Opt. 2012. 17(5), 057006, 1-8.
15. Belozertseva L.A., Voronina E.N., Kokh N.V., Tsvetovskaya G.A., Momot A.P., Lifshits G.I., Filipenko M.L., Shevela A.I., Vlassov V.V. Personalized approach of medication by indirect anticoagulants tailored to the patient-Russian context: what are the prospects? The Epma J. 2012. 3(1), 10.
16. Fomenko N.V., Borgoyakov V.Yu., Panov V.V. Genetic features of Borrelia miyamotoi transmitted by Ixodes persulcatus. Molecular Genetics, Microbiology and Virology. 2011. 26(2), 60-65.

During 2011-2013 Laboratory studies were supported by the Grants of RFBR, Integration projects of the Presidium of SB RAS, projects of the Presidium of SB RAS, grants of the Ministry of education and science of the Russian Federation.

• Automatic biochemical analyser ABX Pentra-400 (France);
• electrolyte analyserAVL-9180 (Roche, Switzerland);
• glycated hemoglobin analyser DS5 Glycomat (Drew Scientific, UK);
• hematology automatic analyser Hemolux-19 (China);
• a spot photometer Multiscan (Finland);
• auto immunechemifluorescent analyser Architect Plus (Abbott, USA);
• automatic immunoassay analyser Alisei (Italy);
• coagulometer Amelung (Germany);
• Biola aggregometer (Russia);
• automatic coagulometer Sysmex-560 (Japan);
• Real Time cycler Rotor-Gene (Corbett research, Australia);
• Real Time cycler CFX-96 (Bio Rad, USA).