Laboratory of Stem Cell

Prof.
Suren M. Zakian
D.Biol.Sci.

Phone: 7 383 363 49 37, Email

• Development of technologies, which control regenerative processes using stem cells and biocompatible polymers.
• Investigation of distribution and differentiation of autologous stem cells after their introduction into the organism.
• Study of possible use of genetic and cellular approaches to diagnostics, prevention and treatment of thromboembolic disorders of cardiovascular disease to reduce early mortality and disability in the working-age population.

• Regeneration processes in site of damaged bone of rat bottom jaw after introduction of autologous mesenchymal stem cells of bone marrow origin (AMSCBMO) in suspension were studied. Formation of red bone marrow in bone callosity occurs much earlier with use of AMSCBMO than in natural course of reparation. Specified changes progress during all time of observation and are evidence of accelerated development of regenerative processes in bone tissue. [Maiborodin I.V. et al., Cell Technologies in Biology and Medicine. 2011. 30, 123; Maiborodin I.V. et al., Cell Technologies in Biology and Medicine. 2011. 4, 215; Maiborodin I.V. et al., Bulletin of Experimental Biology and Medicine. 2011. 152(1), 112].
• Rresults of introduction of autological mesenchymal stem cells from bone marrow (AMSCBM) with trancfected GFP gene in rat uterus scar were studied via light microscopy. Large groups of blood vessels with cellular elements of blood inside were presented in 1 week after introduction of AMSCBM in formed scar on right side (2 months after ligation), such groups of vessels have not been found in uterus scar on opposite side. Bright luminescence in vessel endothelium and adventitium of uterus horn scar only on the party of injection AMSCBM was found during research of unpainted sections in reflected ultra-violet light. Thus, after introduction of AMSCBM, they form blood vessels as result of a differentiation in endotheliocytes and pericytes. GFP gene expresses not only in vessel endothelium, but also in their external layers specifies that AMSCBM differentiation is possible both in endotheliocytes and in pericytes. [Maiborodin I.V. et al., Morphology. 2010. 138(6), 47; Maiborodin I.V. et al., Bulletin of Experimental Biology and Medicine. 2010. 150(12), 705; Maiborodin I.V. et al., J. Biomedical Science and Engineering. 2011. 4(3), 164].
• Reaction of rat tissues at different stages after intraperitoneal implantation of materials from biodegraded polyhydroxyalkanoates (PHA) was studied via light microscopy methods. It was revealed that active adhesive process leads to formation of fibrous solderings between PHA and intestines after PHA implantation in peritoneum. Incapsulation by thick fibrous capsule occurs if PHA is used in form of films. Extensive granuloma of foreign body with perifocal inflammation and sclerosis of surrounding tissues are formed as a result of PHA implantation as ultrathin fibres. Polymer fragmentation and phagocytosis by macrophages with formation of giant cells of foreign body occurs in these granulomas. Materials prepared from PHA are not biodegradable and behave in organism tissues just like other foreign bodies. [Maiborodin I.V. et al., Morphology. 2010. 139(2). 62].
• Method for treating of skin striae based on cellular technologies is developed. [Shevela A.I. et al., Patent RF N.2407555, 2009].
• It is proved that autologous multipotent mesenchymal stem cells of bone marrow origin are involved in restoration of tissue blood flow in trombosed main vein fragment via recanalization of thrombi or the obliteration of thrombosed vessels and growth of new ones. [Maiborodin I.V. et al., Phlebology. 2012; Maiborodin I.V. et al, Morphology. 2012].

1. Mayborodin I.V., Shevela A.I., Morozov V.V., Novikova Ya.V., Matveeva V.A., Drovosekov M.N., Barannik M.I., Marchukov S.V., Kuznetsova I.V. Features of angiogenesis after implantation of polyhydroxyalkanoate films with adsorbed multipotent stromal stem cells of bone marrow origin. Morphology. 2013. 143 (1), 41-47.
2. Mayborodin I.V., Morozov V.V., Novikova Ya.V., Matveeva V.A., Artemjeva L.V., Matveev A.L., Khomenyuk S.V., Marhukov S.V. Introduction of mesenchymal stem cells near the thrombosed vein in the experiment promotes angiogenesis in granulations. Phlebology. 2013. 7(1), 10-16.
3. Medvedev S.P., Smetanina M.A., Shevchenko A.I., Zakharova I.S., Malakhova A.A., Grigorjeva E.V., Dement’eva E.V., Alexandrova M.A., Poltavtseva R.A., Veryasov V.N., Filipenko M.L., Sukhih G.T., Pokushalov E.A., Zakian S.M. Characterization of induced pluripotent stem cells with DNA-microarray. Cell Technologies in Biology and Medicine. 2013. 1, 3-10.
4. Pavlova S.V., Perovskii P.P., Chepeleva E.V., Malakhova A.A., Dementyeva E.V., Pokushalov E.A., Sukhih G.T., Zakian S.M. Characterization of cardiac cell culture, obtaned from myocardium explant. Cell Technologies in Biology and Medicine. 2013. 3, 132-140.
5. Morozov V.V., Novikova Y.V., Shevela A.I., Novak E.V. Recovery of tissue blood flow in rats after administration of multipotent mesenchymal stromal cells in the thrombosed vein. Cell Transplantation and Tissue Engineering. 2012. VI(2), 35-36.
6. Orishchenko K.E., Pavlova S.V., Elisafenko E.A., Sherstyuk V.V., Prinz A.V., Shevchenko A.I., Dementyeva E.V., Zakian S.M. A Regulatory Potential of the Xist Gene Promoter in Vole M. rossiaemeridionalis. PloS ONE. 2012. 7(5), e33994.
7. Meisner L.L., Lotkov A.I., Meisner S.N., Matveyeva V.A., Artem’eva L.V., Matveyev A.L. Effect of silicon, titanium, and zirconium ion implantation on niti biocompatibility. Advances in Materials Science and Engineering. 2012. 2012(N706094), 16.
8. Psakh’e S.G., Meisner L.L., Matveyeva V.A., Artem’eva L.V., Meisner S.N., Lotkov A.I., Matveyev A.L. The effect of chemical composition and roughness of titanium nickelide surface on proliferative properties of mesenchymal stem cells. Inorganic Materials: Applied Research. 2012. 3(2), 135-144.
9. Maiborodin I.V., Shevela A.I., Matveeva V.A., Kolesnikov I.S., Drovosekov M.N., Toder M.S., Perrin T.V. The use of autologous mesenchymal bone marrow stem cells absorbed in fibrin clot for the regeneration of injured lower jawbones in rats. Cell. Ther. Transplan. 2012. 3(10), e.000101.
10. Maiborodin I.V., Morozov V.V., Novikova Y.V., Matveeva V.A., Artemyeva L.V., Matveev A.L., Homenyuk S.V., Marchukov S.V. The morphological results of the introduction of stromal stem cells of bone marrow origin in the thrombosed vein in the experiment. Morphology. 2012. 142(4), 54-61.
11. Morozov V.V., Mayborodin I.V., Novikova Y.V., Matveeva V.A., Artemyeva L.V., Matveev A.L., Volkov A.M. Recovery of tissue blood flow in rats after administration of mesenchymal stem cells in the thrombosed vein. Pathology of circulatory and cardiac surgery. 2012. 4, 89-96.
12. Kuznetsova I.V., Shevela A.I., Morozov V.V., Novikova Y.V., Marchukov S.V., Sevostyanova K.S., Mayborodin I.V. Experimental models of venous thrombosis and the possibility of cell-based technologies for the correction of thrombotic conditions. Phlebology. 2012. 6 (1), 43-47.
13. Maiborodin I., Yakimova N., Matveeva V., Shevela A., Maiborodina E., Pekareva E., Tkachuk O. Angiogenesis in rat uterine scar after introduction of autological mesenchymal stem cells of bone marrow origin. J. Biomedical Science and Engineering. 2011. 4(3), 164-172.
14. Maiborodin I.V., Shevela A.I., Kolesnikov I.S., Drovosekov M.N., Toder M.S., Shevela A.A. Brombin A.I., Beregovoy E.A. Coast Features of stem cells application for regeneration of bone and cartilage. Cell Technologies in Biology and Medicine. 2011. 3, 123-131.
15. Maiborodin I.V., Shevela A.I., Kolesnikov I.S., Drovosekov M.N., Toder M.S., Shevela A.A., Brombin A.I., Beregovoy E.A. Peculiarities of using stem cells for regeneration of the bone and cartilage tissue. Bulletin of Experimental Biology and Medicine. 2011. 152(1), 112-119.
16. Maiborodin I.V., Matveeva V.A., Kolesnikov I.S., Drovosekov M.N., Toder M.S., Shevela A.I. Regeneration of red bone marrow in rat lower jaw after transplantation of mesenchymal stem cells into the site of injury. Bulletin of Experimental Biology and Medicine. 2011. 152(4), 528-534.

1. Morozov V.V., Mayborodin I.V., Novikova Ya.V., Matveeva V.A., Shevela A.I. Experimental method for blood flow restoration in trombosed vein fragment. Patent RF N.2490722, 2013.

Interdisciplinary integration projects of the Presidium of SB RAS (2012-2014)

• N.121 «Comparative studies of articulatory bases of indigenous peoples of Siberia by methods of magnetic resonance imaging, digital radiography and laryngography »
• N.142 «Remote monitoring of the cardiovascular activity of the person on the basis of miniature wireless sensors and individual funds with built-in mobile computing facilities»

Partner integration projects of the Presidium of SB RAS (2012-2014)

• N.40 «Development of new approaches in medical functional diagnostics based on the precise registration of the dynamic responses of blood vessels and the thermal response by thermal imagers of new generation»
• N.55 «Creation of a panel of isogenic induced pluripotent stem cells carrying mutations associated with the development of various forms of amyotrophic lateral sclerosis»

Program of the Presidium of RAS N.5 “Basic science for medicine» (2013-2015)

• N.22 «Application of cellular technology for correction of venous thrombosis in the experiment»
• N.25 «Molecular-genetic aspects of varicose veins pathogenesis of the lower extremities».

• Fluorescent Microscope Axio Imager M1 (Zeiss);
• cryostat microtome HM-550 (Zeiss), HM 340 rotary microtome (Zeiss);
• histological unit carousel STP 120 (Zeiss).